|關於the organization of the same name，參見The Institute。|
- 合成人征用官可以在底层补给/装备供应处找到。他整周全天开放并售卖独特的电浆步枪试作型 18-A、传奇合成人盔甲，如果玩家接受了遭到学院驱逐的任务则无法获取以上装备。
一个带有多个工作站和一个用以测试新武器的射击场的小型实验室。这里开发、测试并创新学院武器用以大量武装学院的合成人步兵与追猎者。This is also where they created the experimental 合成人传送手雷, which will automatically teleport in multiple synths to fight for the player where thrown.
- The advanced systems laboratory contains the entrance to the reactor room.
- Madison Li can be found here.
- Evan Watson, who begins the quest Appropriation, can be found here.
- Shaun can be found here after meeting with Father.
The reactor room is only accessible through the Advanced Systems division after completing the quest Mass Fusion for the Institute or in The Nuclear Option quest for the Brotherhood of Steel, Minutemen or Railroad. Upon entering, there is a long sloped hallway that leads to a larger room with the reactor in the middle, accessible by catwalk. Depending on which faction the Sole Survivor chooses there will be a change in the events that take place here.
- Enemied Institute
If the player character decides to destroy the Institute they will be greeted by three hostile laser turrets upon entering the sloped hallway. In the main reactor room there are multiple synths, Institute scientists and two more laser turrets. Three legendary synths, Z4K-97B, XPN-20A and A-2018 are present. The player character needs to plant the fusion pulse charge in the reactor to sabotage it, which leads to completely erasing the Institute off the face of the Earth with a massive nuclear explosion. Once the agitator is planted the player character is teleported out and can not return.
- Allied Institute
If the player character decides to join the Institute they will be called to the reactor room to insert the beryllium agitator into the reactor, no turrets will be hostile and the room with be filled with scientists. By doing this the Institute will be propelled into the future with enough power to sustain them indefinitely.
Public Works maintenance area
This area is accessed from an underwater pipe due south of Ticonderoga, which leads into a small flooded underground room. There is a terminal-locked security gate in the northeast corner, leading to a small area containing a steamer trunk with leveled loot.
An old and rundown section of the Institute that appears to have been sealed off for many years. The player will only travel through this area when progressing through the quest The Nuclear Option. It's inhabited by many hostile synths and four laser turrets, which can be deactivated using a terminal. There are multiple conveyor belts and pieces of machinery that appear to have been used to assemble protectrons. On the main assembly floor there's a prototype sentry bot which can be activated with the robotics terminal. It will fight the synths that are on the assembly floor, but will also be hostile to the player. The exit is through a circular hatch in the back of the main assembly floor, which will lead to the Bioscience division.
A very small area accessible only by an elevator in the main atrium. It contains a storage room and heavy construction equipment being used to expand the Institute's infrastructure.
N.B. This loot will become unavailable if you are banished from the Institute, or after completing The Nuclear Option for any of the three factions.
- 全像卡帶 先進系統部門筆記在西邊螺旋梯上去最高層梨博士的房間。
- 全像卡帶 部長紀錄#108在部長的休息區。有兩層樓的那棟，在樓上的瓦楞紙箱內。
- 全像卡帶 部長紀錄#52在理事會的會議室。
- 尚恩的終端機密碼 - 理事室桌上，用來解鎖其終端。
- 三卷加入鐵路全像卡帶。兩卷在霍隼的房間垃圾桶內，另一卷在the room top north in the concourse的垃圾桶。
- Three ice cold Nuka-Colas in the cafeteria on the bottom floor.
- An ice cold Nuka-Cherry can be found in Allie Filmore's room.
- Every Institute-unique junk item contains at least some aluminum, and dozens if not hundreds of items can be found throughout the facility.
- 公告腳本 - 啟動任務期間獲得。
- 黃色學院部門主管袍 - 艾莉·菲爾摩身上。
- 橘色學院部門主管袍 - 核子家庭任務後出現在理事室。
- 父親大人的實驗袍 - 父親大人身上， 核子家庭任務後出現在理事室。
- Liam's glasses - Reward for the quest Plugging a Leak, given by siding with Liam Binet.
- 實驗型血清 - 在存放超級變種人大缸的房間，on a counter in the southeast corner.
- 全像卡帶 布萊恩·維吉爾個人紀錄0176 - 同上，在西側終端旁桌上。
- 全像卡帶 FEV研究報告 (InstituteHolotapeFEVLab01) - 同上，在缸槽前面的控制台上。
- 全像卡帶 FEV研究報告 (InstituteHolotapeFEVLab02, 一樣名稱但內容不同) - 同上，另一個控制台上。
- 穿刺管鉗改造配件 - 在儲藏間架上。
- 帶鉤管鉗改造配件 - 同上，另一個架上。
- 五枚合成人轉送手榴彈 on a shelf along the northwest wall.
- 全像卡帶 先進系統部門筆記 (和梨博士房間的卡帶一樣名稱但內容不同)在梨博士的終端旁。
- 反應爐終端機密碼 - 核子手段任務期間於Z4K-97B和A-2018身上取得，用於學院反應爐前的終端機。
- 4N1M4L的實驗記錄 - 在通往有合成人的維護區走廊，右側打開安全門進去到底的房間，在機器人部終端機裡。只有在義勇兵的核子手段任務才會抵達。
- Three cryogenic grenades, the only three fixed ones in the game.
- 全像卡帶 植入更新階段#7在次層21-D，要從學院南邊的儲藏室裡搭電梯進入。卡帶在儲藏間底部架上，從入口近來中間直走，就在椅子旁邊。
- A House Divided
- Airship Down
- Banished from the Institute
- Building a Better Crop
- End of the Line
- From Within
- Inside Job
- Mankind - Redefined
- Mass Fusion
- Nuclear Family
- Outside the Wire
- Pest Control
- Plugging a Leak
- Political Leanings
- Powering Up
- Synth Retention
- The Battle of Bunker Hill
- The Nuclear Option
- Underground Undercover
- Virgil's Cure
- Forbidden Knowledge
- Unlike all other locations in the game, fast traveling to or from this location only passes one minute of in-game time regardless of distance traveled. This can make the Institute a very useful fast-travel slingshot when doing quests that are time-sensitive. In addition, a teleport effect will be visible after arrival.
- Companions will not be able to travel with you to the Institute, with the sole exception of X6-88.
- The resident's name is on each door. If it is slid open (hidden) just close the doors to reveal the name of the occupant.
- Father will remain in the same area as you have found him.
- X6-88 will on occasion be bugged, and you may find it difficult to find him. Check along the shores of Libertalia, as he seems to favor staying there even when he says that he will be returning to the Institute.
- Based on the player's decisions, Institute scientists will have varied dialogue.
- Bioscience holds the location of the only two synthetic gorillas. These gorillas may be teleported to assist in the fight against the Brotherhood in the quest Airship Down.
- When entering the Institute with the Railroad, one may have trouble finding the network scanner, which is currently an unsolvable bug. Console commands can bring the item into the player's inventory.
- After becoming enemies with the Institute, you cannot obtain the Astoundingly Awesome Tales magazine anymore.
- If the player completes the Institute ending there will be a memorial of Shaun in front of the robotics lab which the player can interact with.
- Binet quarters' terminal mentions synths liking Fancy Lads Snack Cakes, and the danger this could pose to them. This could be a joke about some advertisements, where the hero defeats the villain by offering him similar products -Hostess Fruit Pies is one such example.
- The Public Works maintenance area has no local map for the area past the grate needing to be opened with a keypad during The Nuclear Option (Minutemen route.)
- X6-88. [已验证] Before and even after the player has become the director, if they are caught using Madison Li's terminal, all the institute NPCs will become hostile, including
- Silver Shroud Radio can be received in the Old Robotics section, if the radio station is active during The Nuclear Option.[已验证]