Mankind - Redefined: Ayo is vocally against Father's announcement to appoint the Sole Survivor as his successor, but Father's words remain final.
Plugging a Leak: Ayo is trying to find out how synths keep managing to escape despite his efforts to prevent it. This quest is only available if the Sole Survivor has not completed Tradecraft for the Railroad. Otherwise Ayo is too suspicious of their Railroad ties to bring it up.
Political Leanings: Ayo wants the Sole Survivor to go and collect a report from a certain synth agent in a high profile position.