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Gene may refer to:
- Characters:
- Gene (Fallout 4), a dog breeder randomly encountered in Fallout 4.
- Gene (Atlantic City), a chemist for the Lombardi Family of Atlantic City in the Fallout 76 update America's Playground.
- Item:
- Gene's invoice, a paper note in the Fallout 76 update Boardwalk Paradise.
- Real-life people:
- Gene Vassilaros, a director who worked as a voiceover director on Fallout 76.
- Gene Armstrong, a bandleader and musician whose song "Lonely Hours" plays in the Fallout TV series.
- Gene Semel, a developer who worked at Interplay Entertainment as the audio director and cinematics mixer on Fallout: Brotherhood of Steel.
- Other:
- Gene projector, a user interface menu and world object used for character creation in Fallout 3, as well as a world object in Fallout: New Vegas.