The Bitter Springs Massacre is an event mentioned in the graphic novel All Roads and referenced many times throughout Fallout: New Vegas.
Background
During All Roads and Fallout: New Vegas, it is revealed that the New California Republic, most notably the 1st Recon unit, massacred a large number of Great Khan women, children and the elderly at Bitter Springs.
The NCR snipers set up on Coyote Tail Ridge and were ordered to fire until they had exhausted their ammunition on the retreating Khans. The stories differ greatly from character to character due to variations in personality and state of mind, as well as their level of involvement in the incident.
The characters with dialogue in the game include Craig Boone, Manny Vargas, Papa Khan, Major Dhatri, Bitter-Root, Melissa Lewis and Oscar Velasco.
Craig Boone's account
A number of Khans were tracked to Bitter Springs by the NCR many years ago, when the NCR were first expanding their role in the Mojave Wasteland. The NCR believed it to be the Khans' base of operations, and sent a large detachment of troops to bring the raids to an end. At least one company of scout/snipers from the 1st Recon Battalion, Craig Boone included, were sent to cut off escape at Bitter Springs' only other exit. Little did the NCR realize that Bitter Springs was primarily the home of Khan families, and not (as NCR command believed) a bastion of resistance. When the Khans were then pushed out, 1st Recon was ordered to fire on the ones retreating from the area. Radio contact with the NCR was sketchy at best, command not understanding that the situation involved women, children, and elderly. Their superiors ordered them to "fire until you're out of ammunition." 1st Recon obeyed and what happened at Bitter Springs has had a large psychological impact on Boone, motivating him to leave the New California Republic Army behind him.
Chance's account
The history through the eyes of Chance differs from that of Boone, in that the NCR is portrayed as being just as brutal as Caesar's Legion when it comes to pursuing their political agenda for a given area. There was no miscommunication with command, and the NCR soldiers simply rode into the town and opened fire. 1st Recon is not seen at all during Chance's flashback. It had a significant psychological impact on Chance, driving him to the use of drugs and leaving him mute.
Papa Khan's account
Papa Khan states that the Great Khans had been flouting the NCR's demands, carrying out raids under the assumption that NCR's threats of retaliation were simply bluffs. Tensions rose higher and eventually the NCR tracked what they believed was a large raiding party back to Bitter Springs. Having been harassed continuously by the Khans, the NCR was looking to settle a score and did not expect Bitter Springs to be anything but a raider camp. Fueled by anger and frustration, goaded by the Great Khan's audacity and lacking appropriate intel, NCR command ordered the attack on the settlement to disastrous consequence.
Bitter Root's account
Bitter-Root account of the events differs drastically from that of other testimonies. As an ex-Great Khan, he had the chance to see the civilians involved in the massacre. He describes them (including his parents, who were Great Khans) as sadistic murderers who attacked civilians and NCR personnel for fun, and even his own father forced Bitter-Root to shoot NCR civilians while he was still a kid. His testimony depicts an image that shows the attack at Bitter Springs not so much as a massacre of civilians, but as a battle between superior NCR forces and poorly armed and organized Great-Khans raiders who happened to be women and elderly (he also notes that even Khan children had been trained to kill, and that he himself had been forced to kill civilians as a child by his father). But given his adoptive father's stance, it's possible that Bitter-Root's recollection is untrustworthy due to his youth at the time and Dhatri's own prejudice.
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