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Fallout Interactive Experience
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The Audio Sample - Giant Tarantula, is a holotape in the Fallout: New Vegas add-on Old World Blues, which can be given to Blind Diode Jefferson, who will use it to modify the sonic emitter to Sonic emitter - tarantula.
Location
Located upstairs in the leftmost room of house #00 in Higgs Village.
Transcript
This holotape contains an audio sample entitled, "Giant Tarantula Attack." It can be used to modify the sonic emitter weapon at the Sink's Jukebox.